1XRB

S-adenosylmethionine synthetase (MAT, ATP: L-methionine S-adenosyltransferase, E.C.2.5.1.6) in which MET residues are replaced with selenomethionine residues (MSE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 62 2 2
Unit cell lengths	128.900, 128.900, 139.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 3.000
R-factor	0.188
Rwork	0.188
R-free	0.26500
RMSD bond length	0.015
RMSD bond angle	25.800 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	3.000
Rmerge	0.069 *
Total number of observations	62929 *
Number of reflections	13507 *
Completeness [%]	97.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7 *	26 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	potassium phosphate	100 (mM)
2	1	1		10 (mM)
3	1	1	ammonium sulfate	33 (%(v/v))
4	1	1	protein	10 (mg/ml)