1XR8
Crystal Structures of HLA-B*1501 in Complex with Peptides from Human UbcH6 and Epstein-Barr Virus EBNA-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-5 |
| Synchrotron site | MAX II |
| Beamline | I911-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.96900 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.582, 81.891, 110.408 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.437 - 2.300 |
| R-factor | 0.173 |
| Rwork | 0.169 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a1m |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.889 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.440 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Total number of observations | 10859 | |
| Number of reflections | 19549 | |
| <I/σ(I)> | 5.1 | 1.9 |
| Completeness [%] | 93.2 | 96.29 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.2M MgAc4H20, 0.1M Na-cacodylate, pH 6.5, 20% w/v PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
