1XQV

Crystal structure of inactive F1-mutant G37A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-02-11
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	55.280, 57.730, 82.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.300
R-factor	0.242
Rwork	0.242
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mtz
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	MOSFLM
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.400	2.110
High resolution limit [Å]	2.000	2.000
Number of reflections	16574
<I/σ(I)>	10.2
Completeness [%]	98.8	68.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	PEG 6000, Bis-Tris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K