1XQF
The mechanism of ammonia transport based on the crystal structure of AmtB of E. coli.
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-31 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9797 |
Spacegroup name | P 63 |
Unit cell lengths | 111.800, 111.800, 86.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 1.800 |
R-factor | 0.18196 |
Rwork | 0.181 |
R-free | 0.20755 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.121 |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 96.670 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 54526 | |
<I/σ(I)> | 13.2 | 2 |
Completeness [%] | 95.6 | 98.5 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | PEG 550, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |