1XQE
The mechanism of ammonia transport based on the crystal structure of AmtB of E. coli.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-05-28 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9797 |
Spacegroup name | H 3 |
Unit cell lengths | 116.632, 116.632, 130.543 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.000 - 2.100 |
R-factor | 0.16051 |
Rwork | 0.159 |
R-free | 0.18603 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | The same structure in the absence of ammonium |
RMSD bond length | 0.012 |
RMSD bond angle | 1.236 |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.060 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 38583 | |
<I/σ(I)> | 13.6 | 2.5 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 5.8 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | PEG 550, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |