1XQ6

X-ray Structure of Gene Product from Arabidopsis Thaliana At5g02240

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-09
Detector	MAR CCD 165 mm
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	46.750, 69.799, 83.828
Unit cell angles	90.00, 96.01, 90.00

Refinement procedure

Resolution	23.570 - 1.800
R-factor	0.221
Rwork	0.218
R-free	0.27760
Structure solution method	MAD
RMSD bond length	0.020
RMSD bond angle	1.833
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.06)
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	23.565	100.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.043	0.021	0.693
Total number of observations		5275	4811
Number of reflections	52253
<I/σ(I)>	19.7		2.67
Completeness [%]	93.9	92.6	87.3
Redundancy	5.2		5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	10 MG/ML PROTEIN, 28 PERCENT PEG 4K, 0.034 M SODIUM MALONATE, 0.10 M BISTRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	10 MG/ML PROTEIN, 28 PERCENT PEG 4K, 0.034 M SODIUM MALONATE, 0.10 M BISTRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K