1XPR
Structural mechanism of inhibition of the Rho transcription termination factor by the antibiotic 5a-formylbicyclomycin (FB)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Wavelength(s) | 1.100 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 210.526, 109.679, 160.046 |
Unit cell angles | 90.00, 108.16, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.150 |
R-factor | 0.28389 |
Rwork | 0.274 |
R-free | 0.29582 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.081 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.230 |
High resolution limit [Å] | 3.150 | 3.150 |
Number of reflections | 49136 | |
Completeness [%] | 86.5 | 76.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | PEG 3350, Na Cacodylate, NaCl, glycerol, Na DihydrogenPhosphate, pH 6.5, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3350 | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | glycerol | ||
4 | 1 | 1 | NaH2PO4 | ||
5 | 1 | 1 | sodium Cacodylate | ||
6 | 1 | 2 | PEG 3350 | ||
7 | 1 | 2 | NaCl | ||
8 | 1 | 2 | glycerol | ||
9 | 1 | 2 | NaH2PO4 |