1XPH
Structure of DC-SIGNR and a portion of repeat domain 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-28 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.0395 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.233, 54.887, 62.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.400 - 1.410 |
| R-factor | 0.17698 |
| Rwork | 0.176 |
| R-free | 0.19295 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k9j |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.366 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.400 | 1.480 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Number of reflections | 25340 | |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 97.5 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100mM MgCl2, 100mM Na Cacodylate, 12% PEG 3000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
