1XP8
Deinococcus radiodurans RecA in complex with ATP-gamma-S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 61 |
| Unit cell lengths | 111.380, 111.380, 67.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.650 - 2.500 |
| Rwork | 0.228 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2reb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.650 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.084 | 0.411 |
| Number of reflections | 16644 | |
| <I/σ(I)> | 12.2 | 3.6 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 295 | PEG 4000, ammonium acetate, calcium chloride, Mes, pH 8.0, VAPOR DIFFUSION, temperature 295K |
