1XP7
HIV-1 subtype F genomic RNA Dimerization Initiation Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 27.380, 116.190, 94.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 5206 |
| <I/σ(I)> | 33 |
| Completeness [%] | 99.6 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 310 | PEG8000, Ammonium sulfate, MgCl2, Na Cacodylate, spermine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG8000 | ||
| 10 | 1 | 2 | spermine | ||
| 2 | 1 | 1 | Ammonium sulfate | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | Na Cacodylate | ||
| 5 | 1 | 1 | spermine | ||
| 6 | 1 | 2 | PEG8000 | ||
| 7 | 1 | 2 | Ammonium sulfate | ||
| 8 | 1 | 2 | MgCl2 | ||
| 9 | 1 | 2 | Na Cacodylate |
