1XOC
The structure of the oligopeptide-binding protein, AppA, from Bacillus subtilis in complex with a nonapeptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 120.955, 90.779, 54.719 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.570 - 1.550 |
| R-factor | 0.17703 |
| Rwork | 0.176 |
| R-free | 0.20593 |
| Structure solution method | MOLECULAR REPLACEMENT + MIR |
| Starting model (for MR) | 1dpe |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.469 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.081 | 0.788 |
| Number of reflections | 88121 | |
| <I/σ(I)> | 18.5 | 1.6 |
| Completeness [%] | 97.3 | 72.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG8000, zinc acetate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
