1XO7
Crystal structure of cyclophilin from Trypanosoma cruzi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.314, 62.142, 64.638 |
| Unit cell angles | 109.55, 104.21, 104.92 |
Refinement procedure
| Resolution | 56.800 - 1.610 |
| R-factor | 0.18 |
| Rwork | 0.179 |
| R-free | 0.19774 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1DYW.pdb |
| RMSD bond length | 0.038 |
| RMSD bond angle | 2.610 |
| Data reduction software | ELVES |
| Data scaling software | ELVES |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.800 | 1.700 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Number of reflections | 80854 | |
| <I/σ(I)> | 7.2 | 2 |
| Completeness [%] | 93.4 | 89.4 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | Ammonium Phosphate monobasic, Sodium Acetate, PEG8000, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 25K, temperature 298K |
