1XNW
Acyl-CoA Carboxylase Beta Subunit from S. coelicolor (PccB), apo form #2, mutant D422I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-09 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.850, 219.492, 135.691 |
Unit cell angles | 90.00, 102.99, 90.00 |
Refinement procedure
Resolution | 49.050 - 2.600 |
R-factor | 0.2611 |
Rwork | 0.261 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | homology model of PccB |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.050 | 2.650 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 139417 | |
<I/σ(I)> | 8.5 | 1.4 |
Completeness [%] | 100.0 | 99.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 35% MPD, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |