1XNS
Peptide trapped Holliday junction intermediate in Cre-loxP recombination
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 106.078, 121.529, 177.799 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.670 - 2.800 |
| R-factor | 0.19965 |
| Rwork | 0.196 |
| R-free | 0.26541 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1crx |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.713 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.670 | 2.880 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 26172 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 20 mM ACETATE BUFFER PH 5.0, 25% MPD, 20 mM CACL2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
