1XNF
Crystal structure of E.coli TPR-protein NlpI
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-18 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9785, 0.9795, 0.9500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.351, 81.654, 136.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.840 - 1.980 |
| R-factor | 0.17122 |
| Rwork | 0.169 |
| R-free | 0.20577 |
| Structure solution method | MAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.854 |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.100 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.049 | 0.355 |
| Number of reflections | 48199 | |
| <I/σ(I)> | 40.4 | 4.8 |
| Completeness [%] | 83.6 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | HEPES, MgCl2, PEG400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
