1XMX

Crystal structure of protein VC1899 from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-25
Detector	CUSTOM-MADE
Wavelength(s)	0.9794
Spacegroup name	P 21 21 2
Unit cell lengths	66.549, 71.439, 90.096
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.17261
Rwork	0.168
R-free	0.25371
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.777
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.139	0.739
Number of reflections	24106
<I/σ(I)>		1.358
Completeness [%]	92.5	59.3
Redundancy		3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	296	HEPES, Magnesium, Formate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K, temperature 23K