1XMQ
Crystal Structure of t6A37-ASLLysUUU AAA-mRNA Bound to the Decoding Center
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9393 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 400.723, 400.723, 175.088 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 99.000 - 3.000 |
Rwork | 0.222 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | empty 30S |
RMSD bond length | 0.006 |
RMSD bond angle | 1.201 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 263974 | |
<I/σ(I)> | 6.5 | 2 |
Completeness [%] | 97.4 | 91.3 |
Redundancy | 29.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | MPD, magnesium chloride, potassium chloride, ammonium chloride, MES-KOH, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
10 | 1 | 2 | ammonium chloride | ||
2 | 1 | 1 | magnesium chloride | ||
3 | 1 | 1 | potassium chloride | ||
4 | 1 | 1 | ammonium chloride | ||
5 | 1 | 1 | MES-KOH | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | MPD | ||
8 | 1 | 2 | magnesium chloride | ||
9 | 1 | 2 | potassium chloride |