1XMD

M335V mutant structure of mouse carnitine octanoyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9790
Spacegroup name	H 3
Unit cell lengths	164.000, 164.000, 159.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.000 - 2.100
Rwork	0.196
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	COMO
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	92430
Completeness [%]	99.9	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	277	100mM Hepes, 62% v/v MPD, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K