1XMB
X-ray structure of IAA-aminoacid hydrolase from Arabidopsis thaliana gene AT5G56660
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2004-08-01 |
Detector | APS-1 |
Wavelength(s) | 0.96411 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 75.264, 75.264, 130.880 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.210 - 2.000 |
R-factor | 0.159 |
Rwork | 0.157 |
R-free | 0.20390 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.587 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.06) |
Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 24.210 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.043 | 0.024 | 0.334 |
Total number of observations | 3132 | 2939 | |
Number of reflections | 29672 | ||
<I/σ(I)> | 22 | 3.8 | |
Completeness [%] | 99.9 | 98.8 | 99.9 |
Redundancy | 8.4 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 10 mg/mL PROTEIN, 0.080 M MAGNESIUM SULFATE, 14 % PEG 1500, 0.100 M CHES, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 10 mg/mL PROTEIN, 0.080 M MAGNESIUM SULFATE, 14 % PEG 1500, 0.100 M CHES, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |