1XM8

X-RAY STRUCTURE OF GLYOXALASE II FROM ARABIDOPSIS THALIANA GENE AT2G31350

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-14
Detector	ADSC QUANTUM 4
Spacegroup name	P 1 21 1
Unit cell lengths	68.494, 58.782, 69.049
Unit cell angles	90.00, 109.22, 90.00

Refinement procedure

Resolution	33.710 - 1.740
R-factor	0.142
Rwork	0.140
R-free	0.18900
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.746
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.06)
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.720	1.800
High resolution limit [Å]	1.742	1.740
Rmerge	0.079	0.545
Number of reflections	51949
<I/σ(I)>	21.2	4.34
Completeness [%]	97.4	92.9
Redundancy	7.6	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5	293	10 MG/ML PROTEIN, 0.100 SODIUM ACETATE, 16 PERCENT PEG 4K, 10 PERCENT PEG 400, 0.200 GLYCINE, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 4.50