1XM8

X-RAY STRUCTURE OF GLYOXALASE II FROM ARABIDOPSIS THALIANA GENE AT2G31350

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-14
Detector	ADSC QUANTUM 4
Spacegroup name	P 1 21 1
格子定数 [Å]	68.494, 58.782, 69.049
格子定数 [度]	90.00, 109.22, 90.00

精密化法

分解能	33.710 - 1.740
R因子	0.142
Rwork	0.140
R-free	0.18900
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.020
結合角の平均二乗偏差(RMSD) [度]	1.746
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.06)
Refinement software	REFMAC (refmac_5.2.0005)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	33.720	1.800
分解能 [Å] (高)	1.742	1.740
Rmerge_l_obs	0.079	0.545
独立反射数	51949
<I/σ(I)>	21.2	4.34
完全性 [%]	97.4	92.9
冗長性	7.6	7.3

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		4.5	293	10 MG/ML PROTEIN, 0.100 SODIUM ACETATE, 16 PERCENT PEG 4K, 10 PERCENT PEG 400, 0.200 GLYCINE, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 4.50