1XM3

Crystal structure of Northeast Structural Genomics Target SR156

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Spacegroup name	C 1 2 1
Unit cell lengths	144.536, 120.722, 74.244
Unit cell angles	90.00, 108.32, 90.00

Refinement procedure

Resolution	29.820 - 1.800
R-factor	0.212
Rwork	0.212
R-free	0.23700
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.910
High resolution limit [Å]	1.800	1.800
Number of reflections	216070
Completeness [%]		59.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	PEG 3350, magnesium nitrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K