1XLY

X-RAY STRUCTURE OF THE RNA-BINDING PROTEIN SHE2p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-23
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	97.199, 103.647, 56.733
Unit cell angles	90.00, 110.51, 90.00

Refinement procedure

Resolution	19.760 - 1.950
R-factor	0.206
Rwork	0.191
R-free	0.24100
Structure solution method	MIR
RMSD bond length	0.027
RMSD bond angle	2.300
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	37528
<I/σ(I)>	11.1	2.7
Completeness [%]	98.0	97.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	293	PEG 4000, lithium sulfate, tris, beta-octyl-glucoside, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K