1XLQ
Crystal structure of reduced C73S putidaredoxin from Pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 170 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-12-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.070, 66.070, 154.044 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.920 - 1.450 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.20100 |
| Structure solution method | rigid body refinement |
| Starting model (for MR) | 1oqr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.920 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.044 | 0.545 |
| Number of reflections | 60208 | |
| <I/σ(I)> | 28.8 | 2.6 |
| Completeness [%] | 97.9 | 92.7 |
| Redundancy | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | lithium sulfate, sodium citrate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
