1XLP
Structure of oxidized C73S putidaredoxin from Pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 170 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-02 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.220, 66.220, 151.751 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.360 - 2.000 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.22100 |
| Structure solution method | rigid body refinement |
| Starting model (for MR) | 1oqr |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.360 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.132 | 0.674 |
| Number of reflections | 23600 | |
| <I/σ(I)> | 17 | 2.9 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | lithium sulfate, sodium citrate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
