1XLN
Crystal structure of oxidized C73S/C85S putidaredoxin, a [2Fe-2S] ferredoxin from Pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 170 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.166, 75.449, 107.859 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.960 - 2.030 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.23100 |
| Structure solution method | rigid body refinement |
| Starting model (for MR) | 1oqq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.960 | 2.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.078 | 0.372 |
| Number of reflections | 13405 | |
| <I/σ(I)> | 16.4 | 2.7 |
| Completeness [%] | 98.4 | 91.9 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | Ammonium sulfate, sodium citrate, sodium/potassium phosphate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
