1XL6
Intermediate gating structure 2 of the inwardly rectifying K+ channel KirBac3.1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 105.756, 107.532, 89.654 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.850 |
Rwork | 0.270 |
R-free | 0.31000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p7b |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.700 |
Number of reflections | 28510 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 90mM Hepes, 2.5% PEG 8000, 2.5% PEG 4000, 20% PEG 400, 12.5mM magnesium chloride, 42.5mM magnesium acetate, 2.5% glycerol, 14mM Hega-10 , pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |