1XKW
Pyochelin outer membrane receptor FptA from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979530 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.910, 84.540, 162.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.300 - 2.000 |
| Rwork | 0.222 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xkh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.590 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.300 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 71021 | |
| <I/σ(I)> | 15.45 | 3.65 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 4.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 4.5 to 9 % PEG 8K or 10 K, 150 mM (NH4)2S04, 25 % ethylene glycol as cryoprotecting agent, 0.1 M sodium acetate pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K |
