1XKS
The crystal structure of the N-terminal domain of Nup133 reveals a beta-propeller fold common to several nucleoporins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-28 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 84.588, 84.588, 139.048 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.460 - 2.350 |
| R-factor | 0.2 |
| Rwork | 0.196 |
| R-free | 0.23600 |
| Structure solution method | SAD |
| Starting model (for MR) | Model built by SAD data from SeMet derivative |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.167 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 20899 | |
| Completeness [%] | 96.5 | 93.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.7 | 277 | PEG 3350, lithium sulfate, bis-tris, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K, pH 5.70 |
