1XKH
Pyoverdine outer membrane receptor FpvA from Pseudomonas aeruginosa PAO1 bound to pyoverdine
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979413, 0.979632, 0.977801 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.451, 231.342, 121.695 |
| Unit cell angles | 90.00, 104.57, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.600 |
| Rwork | 0.259 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Ferrichrome outer membrane receptor FhuA from Escherichia coli. |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.559 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.700 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 41896 | |
| <I/σ(I)> | 8.46 | 1.14 |
| Completeness [%] | 98.4 | 96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | 13-16% PEG 4000, 20-25% ethylene glycol as cryoprectant in a 0.1M sodium citrate buffer, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
