1XJZ

Crystal Structures of the G139A, G139A-NO and G143H Mutants of Human Heme Oxygenase-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Collection date	2004-03-17
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	54.251, 75.985, 106.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.880
Rwork	0.222
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.010
High resolution limit [Å]	1.880	1.880
Number of reflections	66892
Completeness [%]	95.0	95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	ammonium sulphate, hepes, hexane diol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K