1XJU

Crystal structure of secreted inactive form of P1 phage endolysin Lyz

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Wavelength(s)	0.9
Spacegroup name	P 1 21 1
Unit cell lengths	33.349, 59.786, 76.228
Unit cell angles	90.00, 102.51, 90.00

Refinement procedure

Resolution	46.620 - 1.070
R-factor	0.134
Rwork	0.133
R-free	0.15300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.917
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.110
High resolution limit [Å]	1.070	2.310	1.070
Rmerge	0.057	0.032	0.318
Number of reflections	116001
Completeness [%]	90.2	98.7	51.9
Redundancy	5.6	5.3	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	291.15	sodium acetate, PEG4000, pH 4.5, VAPOR DIFFUSION, temperature 291.15K