1XJ6
Structure of bjFixLH in the unliganded ferrous form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 298 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.900 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.100, 59.915, 59.574 |
Unit cell angles | 90.00, 111.51, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.900 |
Rwork | 0.207 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1drm |
RMSD bond length | 0.008 |
RMSD bond angle | 1.680 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.059 |
Number of reflections | 19749 |
Completeness [%] | 93.7 |
Redundancy | 5.14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | NaCl, Ethylene glycol, TRIS, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |