1XIP
Crystal Structure of the N-terminal Domain of Nup159
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9793, 1.10 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.359, 71.516, 90.728 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.20579 |
Rwork | 0.203 |
R-free | 0.24890 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.302 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 13707 | |
<I/σ(I)> | 17.2 | 2.5 |
Completeness [%] | 97.7 | 93.3 |
Redundancy | 3.5 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 292 | PEG3350, ammonium acetate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |