1XIO
Anabaena sensory rhodopsin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-01-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8984 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 103.095, 116.513, 56.186 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
Rwork | 0.230 |
R-free | 0.25590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jgj |
RMSD bond length | 0.007 |
RMSD bond angle | 1.051 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.097 | 0.511 |
Number of reflections | 23141 | |
<I/σ(I)> | 18 | 2.4 |
Completeness [%] | 99.0 | 93.8 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | cubic lipid phase | 5.6 | 293 | MPD, pH 5.6, cubic lipid phase, temperature 293K |