1XHO
Chorismate mutase from Clostridium thermocellum Cth-682
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 31 |
| Unit cell lengths | 48.201, 48.201, 123.693 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.960 - 2.200 |
| R-factor | 0.198 |
| Rwork | 0.194 |
| R-free | 0.25500 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.103 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.03) |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.057 | 0.167 |
| Number of reflections | 15073 | |
| Completeness [%] | 93.3 | 42.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 291 | 2.5M ammonium sulfate, 0.1M HEPES, pH 7.6, vapor diffusion, temperature 291K, pH 7.60 |
