1XHN
The crystal structure of Cellular Repressor of E1A-stimulated Genes (CREG)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979502, 0.980005, 0.972318 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 106.130, 121.260, 55.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.950 |
| R-factor | 0.16299 |
| Rwork | 0.162 |
| R-free | 0.21314 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.557 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 51850 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | Na acetate, PEG4000, ethylene glycole, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
