1XHG
Crystal structure of a 40 kDa signalling protein from Porcine (SPP-40) at 2.89A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5412 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.750, 66.454, 107.652 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.000 - 2.900 |
| R-factor | 0.17911 |
| Rwork | 0.177 |
| R-free | 0.21765 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1owq |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.015 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 3.000 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Number of reflections | 9476 | |
| <I/σ(I)> | 3.3 | 1.3 |
| Completeness [%] | 90.7 | 87.1 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 25mM tris-HCl, 50mM NaCl, 20% ethanol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
