1XHE

Crystal structure of the receiver domain of redox response regulator arca

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-09-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97896
Spacegroup name	P 65 2 2
Unit cell lengths	86.695, 86.695, 214.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.21488
Rwork	0.213
R-free	0.25647
Structure solution method	SAD
Starting model (for MR)	Peak-Wavelength Derived Phases
RMSD bond length	0.017
RMSD bond angle	1.615
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.536	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	17085
<I/σ(I)>	18.2	2.9
Completeness [%]	98.3	95.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	Ammonium Acetate, MPD, Sodium Citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	Ammonium Acetate, MPD, Sodium Citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K