1XGO

METHIONINE AMINOPEPTIDASE FROM HYPERTHERMOPHILE PYROCOCCUS FURIOSUS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	289
Detector technology	IMAGE PLATE
Collection date	1996-05
Detector	RIGAKU
Spacegroup name	P 62
Unit cell lengths	138.916, 138.916, 63.907
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 3.500
R-factor	0.182
Rwork	0.182
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MONOCLINIC CRYSTAL FORM PDB ENTRY 1XGM
RMSD bond length	0.016
RMSD bond angle	25.210 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.630
High resolution limit [Å]	3.500	3.500
Rmerge	0.119	0.265
Total number of observations	59765 *
Number of reflections	7571
<I/σ(I)>	6.1	1.9
Completeness [%]	84.3	64.9
Redundancy	3.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	2.6	293 *	PROTEIN SOLUTION CONTAINING 16 MG/ML PFMAP, 2 MM COCL2 AND 30 MM L-METHIONINE IN 20 MM POTASSIUM ACETATE AT PH4.5 WAS MIXED WITH EQUAL AMOUNT OF RESERVOIR SOLUTION CONTAINING 10% PEG6000 AND 2.0 M SODIUM CHLORIDE AT PH 2.6, THEN EQUILIBRATED AGAINST RESERVOIR SOLUTION.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	10 (%(v/v))
2	1	reservoir	sodium chloride	2.0 (M)