1XGN
METHIONINE AMINOPEPTIDASE FROM HYPERTHERMOPHILE PYROCOCCUS FURIOSUS
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 289 |
Detector technology | IMAGE PLATE |
Collection date | 1996-06 |
Detector | RIGAKU |
Spacegroup name | P 64 |
Unit cell lengths | 110.980, 110.980, 124.960 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.900 |
R-factor | 0.156 |
Rwork | 0.156 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MONOCLINIC CRYSTAL FORM PDB ENTRY 1XGM |
RMSD bond length | 0.014 |
RMSD bond angle | 24.950 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.030 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.072 | 0.245 |
Total number of observations | 91220 * | |
Number of reflections | 17502 | |
<I/σ(I)> | 12.4 | 2.1 |
Completeness [%] | 90.0 | 62.7 |
Redundancy | 2.81 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 4.5 * | 277 * | Tahirov, T.H., (1997) Acta Crystallogr.,Sect.D, 53, 798. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | 2 (mM) | ||
3 | 1 | drop | potassium acetate | 20 (mM) | |
4 | 1 | reservoir | 2-propanol | 0.6 (%) | |
5 | 1 | reservoir | PEG4000 | 1.6 (%) | |
6 | 1 | reservoir | sodium HEPES | 0.1 (M) |