1XGM
METHIONINE AMINOPEPTIDASE FROM HYPERTHERMOPHILE PYROCOCCUS FURIOSUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | FUJI |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.385, 85.059, 72.705 |
| Unit cell angles | 90.00, 107.71, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.26300 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 24.730 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.048 | 0.260 |
| Total number of observations | 42890 * | |
| Number of reflections | 12285 | |
| <I/σ(I)> | 19.8 | 2.1 |
| Completeness [%] | 80.3 | 51.9 |
| Redundancy | 2.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.5 | 293 * | Tahirov, T.H., (1997) Acta Crystallogr.,Sect.D, 53, 798. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | 1 (mM) | ||
| 3 | 1 | drop | potassium acetate | 10 (mM) | |
| 4 | 1 | drop | ethanol | 10 (%) | |
| 5 | 1 | drop | Tris-HCl | 0.05 (M) | |
| 6 | 1 | reservoir | ethanol | 20 (%) | |
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) |
