1XGM
METHIONINE AMINOPEPTIDASE FROM HYPERTHERMOPHILE PYROCOCCUS FURIOSUS
Experimental procedure
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 283 |
Detector technology | IMAGE PLATE |
Collection date | 1996-06 |
Detector | FUJI |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.385, 85.059, 72.705 |
Unit cell angles | 90.00, 107.71, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
R-factor | 0.181 |
Rwork | 0.181 |
R-free | 0.26300 |
Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 24.730 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.048 | 0.260 |
Total number of observations | 42890 * | |
Number of reflections | 12285 | |
<I/σ(I)> | 19.8 | 2.1 |
Completeness [%] | 80.3 | 51.9 |
Redundancy | 2.95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8.5 | 293 * | Tahirov, T.H., (1997) Acta Crystallogr.,Sect.D, 53, 798. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | 1 (mM) | ||
3 | 1 | drop | potassium acetate | 10 (mM) | |
4 | 1 | drop | ethanol | 10 (%) | |
5 | 1 | drop | Tris-HCl | 0.05 (M) | |
6 | 1 | reservoir | ethanol | 20 (%) | |
7 | 1 | reservoir | Tris-HCl | 0.1 (M) |