1XGE
Dihydroorotase from Escherichia coli: Loop Movement and Cooperativity between subunits
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.582, 78.826, 180.222 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.17018 |
| Rwork | 0.168 |
| R-free | 0.21067 |
| Structure solution method | Refinement |
| Starting model (for MR) | 1j79 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.185 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.056 | 0.256 |
| Number of reflections | 54214 | |
| <I/σ(I)> | 17.4 | 2.8 |
| Completeness [%] | 92.0 | 83.8 |
| Redundancy | 2.5 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 3350, MES, potassium chloride, magnesium chloride, L-dihydroorotate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
