1XG2
Crystal structure of the complex between pectin methylesterase and its inhibitor protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-18 |
Detector | MARRESEARCH |
Wavelength(s) | 0.99 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 90.379, 90.379, 149.095 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 1.900 |
R-factor | 0.19941 |
Rwork | 0.198 |
R-free | 0.23176 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.211 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.082 | 0.338 |
Number of reflections | 56180 | |
Completeness [%] | 97.5 | 92.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | magnesium sulfate, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |