1XG0
High resolution crystal structure of phycoerythrin 545 from the marine cryptophyte rhodomonas CS24
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-19 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.980, 82.764, 89.477 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.860 - 0.970 |
R-factor | 0.10727 |
Rwork | 0.107 |
R-free | 0.12598 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qgw |
RMSD bond length | 0.017 |
RMSD bond angle | 1.809 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.860 | 1.020 |
High resolution limit [Å] | 0.966 | 0.966 |
Rmerge | 0.057 | 0.550 |
Number of reflections | 249226 | |
<I/σ(I)> | 13.4 | 1.8 |
Completeness [%] | 89.7 | 82.1 |
Redundancy | 4.4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |