1XFJ
Crystal structure of protein CC_0490 from Caulobacter crescentus, Pfam DUF152
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-14 |
Detector | BRANDEIS - B4 |
Wavelength(s) | 0.9792, 0.9797, 0.94 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.807, 66.828, 43.170 |
Unit cell angles | 90.00, 110.60, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.750 |
Rwork | 0.180 |
R-free | 0.22000 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.052 | 0.125 |
Number of reflections | 21008 | |
<I/σ(I)> | 15.8 | |
Completeness [%] | 97.8 | 81.4 |
Redundancy | 7.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | PEG 4000, Sodium-acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |