1XFI

X-ray structure of gene product from Arabidopsis thaliana At2g17340

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	110
Detector technology	CCD
Collection date	2004-08-01
Detector	APS-1
Wavelength(s)	0.96411
Spacegroup name	P 1
Unit cell lengths	40.174, 43.280, 52.644
Unit cell angles	74.57, 74.60, 84.04

Refinement procedure

Resolution	49.386 - 1.700
R-factor	0.17156
Rwork	0.169
R-free	0.22130
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.642
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.039	0.037	0.277
Total number of observations		1807	1638
Number of reflections	35346
<I/σ(I)>	23.3	5.23	5.23
Completeness [%]	97.0	98.6	89.5
Redundancy	4.3	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	10 MG/ML PROTEIN, 0.100M HEPPS, 0.020M MAGNESIUM CHLORIDE, 22 PERCENT PEG 8000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8	293	10 MG/ML PROTEIN, 0.100M HEPPS, 0.020M MAGNESIUM CHLORIDE, 22 PERCENT PEG 8000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K