1XFH
Structure of glutamate transporter homolog from Pyrococcus horikoshii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-06-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.10, 1.0711, 0.9918, 1.0721 |
| Spacegroup name | P 61 |
| Unit cell lengths | 116.000, 116.000, 322.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 3.500 |
| R-factor | 0.29086 |
| Rwork | 0.290 |
| R-free | 0.30910 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.545 |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 3.650 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.078 | 0.840 |
| Number of reflections | 30587 | |
| <I/σ(I)> | 7.3 | 0.7 |
| Completeness [%] | 99.0 * | 98 * |
| Redundancy | 6.7 * | 4.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 | 4 * | PEG 1000, Na/K phosphate, citrate, decyl maltoside, pH 4.0, VAPOR DIFFUSION |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7-10 (mg/ml) | |
| 2 | 1 | reservoir | PEG1000 | 14-18 (%) | |
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | citric acid | 50 (mM) | |
| 5 | 1 | reservoir | sodium phosphate | 50 (mM) |
