1XFG
Glutaminase domain of glucosamine 6-phosphate synthase complexed with l-glu hydroxamate
Replaces: 1GMSExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-11-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9300 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.250, 82.400, 85.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.850 |
| R-factor | 0.159 |
| Rwork | 0.159 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gph |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.830 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.053 | 0.262 |
| Number of reflections | 42930 | |
| <I/σ(I)> | 15.2 | 3.2 |
| Completeness [%] | 99.6 | 92.3 |
| Redundancy | 4.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1 M cacodylate, 1 M sodium acetate, 20% PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
