Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1993-11-08 |
Detector | MARRESEARCH |
Wavelength(s) | 0.99 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.380, 82.520, 86.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.1611 |
Rwork | 0.161 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gph |
RMSD bond length | 0.015 |
RMSD bond angle | 1.728 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.000 | 1.820 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.062 | 0.283 |
Number of reflections | 47043 | |
<I/σ(I)> | 14.4 | 3 |
Completeness [%] | 99.9 | 97.1 |
Redundancy | 5.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1 M cacodylate, 1 M sodium acetate, 20% PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |