1XFD
Structure of a human A-type Potassium Channel Accelerating factor DPPX, a member of the dipeptidyl aminopeptidase family
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2004-01-25 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.980, 170.230, 159.316 |
Unit cell angles | 90.00, 92.11, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.000 |
R-factor | 0.2481 |
Rwork | 0.248 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | HOMOLOGY MODEL BASED ON PDB ENTRY 1N1M |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 18.000 |
High resolution limit [Å] | 2.800 |
Rmerge | 0.098 |
Number of reflections | 76240 |
<I/σ(I)> | 15 |
Completeness [%] | 90.6 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.25 | 295 | MES, NaCl, PEG MME 2000, magnesium chloride, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 295K |